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To maintain good pump performance, the system must be kept clean, the quality of the solvents and reagents used must be maintained and the mobile phase must be filtered and degassed before entering the flow path. Preventing pump failure requires a long-lasting effort: 1) Use of high-quality reagents and HPLC-grade solvents will benefit the pump on the column; 2) Mobile phases and solvents are filtered using premium materials prior to use; 3) Mobile phase and solvent Into the process of degassing; 4) each time you start to use the exhaust vent, after work from the pump and column wash buffer or harmful substances; do not allow water and corrosive solvents and other harmful substances stuck in the pump and the column ; 5) Regular replacement of washers, such as online filter rings; and 6) Strict compliance with pump operation manual recommendations. Pumps in good working order should be able to stabilize the baseline on the chromatogram; reproducibility of retention time is good. Fluctuations in isocratic elution are less than 2%. Gradient elution pressure changes should be slow and steady. In order to rule out the failure as soon as possible after the failure, usually should be standing seal, check valve (inlet and outlet), pump head device, all kinds of fittings, fuses and other components, and replacement tools.